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Peasy basic seamless cloning and assembly kit

WebLinearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Basic Cloning Vectors More Plasmid Sets Home Plasmids Basic Cloning VectorspUC19L Show Static … WebJan 12, 2024 · Expression vectors were constructed using pEASY® -Basic Seamless Cloning and Assembly Kit (TransGen Biotech, Beijing, China). The whole coding sequences were amplified from cDNA templates of young fruit of ‘Xindajiubao’ using the KOD-plus-neo high-fidelity enzyme kit (Toyobo, Osaka, Japan).

GeneArt™ Seamless Cloning and Assembly Kit

WebMay 17, 2016 · The PQQ and terminal ubiquinol cytochrome bo 3 oxidase fused expression plasmid was constructed by the pEASY-Uni seamless cloning and assembly kit CU101 (TransGen, China). ... The cDNA was synthesized from the total RNA using a PrimeScript RT Reagent Kit (Perfect Real Time) (Takara) according to the manufacturer’s protocol. WebDec 1, 2024 · Recently a commercially available pEASY -Uni Seamless Cloning and Assembly Kit (TransGen Biotech) has been made based on the technology of Gibson … lick newcastle https://mayaraguimaraes.com

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WebTransGen Biotech pEASY®-Basic Seamless Cloning and Assembly Kit takes advantage of proprietary recombinases and homologous recombination to achieve directional … WebDec 1, 2024 · Recently a commercially available pEASY -Uni Seamless Cloning and Assembly Kit (TransGen Biotech) has been made based on the technology of Gibson Assembly. In this study, we checked ease of construct design, flexibility and efficiency of pEASY -Uni Seamless Cloning and Assembly Kit on multiple DNA fragments of Elaeis … WebAug 9, 2024 · Due to its ability to perform fast and high-density fermentation, Pichia pastoris is not only used as an excellent host for heterologous protein expression but also exhibits good potential for efficient biosynthesis of small-molecule compounds. mckinsey diversity report 2022

pUC19L Sequence and Map - SnapGene

Category:pEASY®-Basic Seamless Cloning and Assembly Kit - TransGen …

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Peasy basic seamless cloning and assembly kit

pEASY-Basic Seamless Cloning and DNA Assembly Kit – CU201

WebGibson was performed by following the manual of pEASY- Basic Seamless Cloning and Assembly Kit (Transgene, CU201). 【4】Endonuclease digestion and ligation The endonucleases and T4 ligase were bought from NEB Company and the experiments were performed by following instructions. 【5】Transformation

Peasy basic seamless cloning and assembly kit

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WebApr 12, 2024 · The subsequent ligation reaction was carried out at 50 °C according to the pEASY-Basic Seamless Cloning and Assembly Kit (TransGen Biotech), after which ligation products were transformed into E. coli DH5α. Positive colonies were screened and plasmid was isolated and sent to Sangon (Shanghai, China) for DNA sequencing. Protein purification WebMar 28, 2024 · The recombinant plasmid pYES2-HcOSC6was obtained by recombining the vector and the target gene fragment with pEASY®-Basic Seamless Cloning and Assembly Kit. The recombinant plasmid pYES2-HcOSC6was transformed into DH5α competent cells and cultured overnight on a LB solid plate containing 100 mg·L-1Amp.

WebpEASY ®-Basic Seamless Cloning and Assembly Kit Cloning Competent Cells Trans 10 Chemically Competent Cell Trans 5α Chemically Competent Cell Trans 109 Chemically Competent Cell Trans 110 Chemically Competent Cell Trans 1-Blue Chemically Competent Cell Trans 2-Blue Chemically Competent Cell Trans 1-T1 Phage Resistant Chemically … WebpEASY® -Basic Seamless Cloning and Assembly Kit 目录号： CU201-02 单 价： ￥620 规格： 20 rxns 60 rxns 数量： - + 说明书 质检报告 立即下单 加入购物车 产品详情介绍 本产品 …

WebDec 1, 2024 · Recently a commercially available pEASY-Uni Seamless Cloning and Assembly Kit (TransGen Biotech) has been made based on the technology of Gibson Assembly. In … WebCloning Vectors 1 Selected products (0/20) Contact supplier Click to select products that you want to inquire about before clicking the Contact supplier button. pEASY-Blunt3 Cloning Kit $92.30 / piece pEASY-T1 Cloning Kit $80.00 - $90.00 / set 1 set (Min. Order) pEASY-T1 Simple Cloning Kit $80.00 - $90.00 / set 1 set (Min. Order)

WebMar 24, 2024 · Based on our transcriptome data, we cloned five type-B LlRR s ( LlRR1, LlRR2, LlRR10, LlRR11 and LlRR12) via RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) and performed phylogenetic analysis, subcellular localization experiments and expression pattern analysis.

Web5 rows · Product Details. This kit takes advantage of proprietary recombinases and homologous recombination ... lick nintendo switch cartridgeWebFast accurate construct design for all major molecular cloning techniques; Validate sequenced constructs using powerful alignment tools; Customize plasmid maps with … mckinsey electric vehicles indiaWebMay 9, 2024 · To develop the plasmids expressing ASFV p32 and p54, we constructed the replicon plasmids pSFV-p32 and pSFV-p54 via homologous recombination using the pEASY ® -Basic Seamless Cloning and Assembly Kit and pSFV-EGFP as described above. mckinsey electric vehicle forecastWebJul 13, 2024 · The fused fragments were inserted into the pRB1028 at the restriction sites Hand III and Bam HI by using the pEASY ®-Basic Seamless Cloning and Assembly Kit (TransGen Biotech, Beijing, China). pRB-secYEG was constructed successfully after DNA sequencing and was electro-transferred into B. thuringiensis BMB171. The positive … licknriff brothers in armsWeb5 rows · Product Details. This kit takes advantage of proprietary recombinases and homologous recombination ... licknriff crazyWebMar 28, 2024 · PDF Hemsleya chinensis is a Chinese traditional medicinal plant, containing cucurbitacin IIa (CuIIa) and cucurbitacin IIb (CuIIb), both of which have... Find, read and cite all the research ... mckinsey electric vehicle index 2022WebOne seamless cloning method is the Gibson Assembly method, originally described by Daniel G. Gibson, of the J. Craig Venter Institute. His exonuclease-based method is … licknriff courses