WebThe fluorometric measurement was performed by the method of Mattingly.5 The Porter-Silber reaction was carried out according to the modification of Peterson2 with the spectrum from 350 m/.c to 450 rnfc being read in the Beckman-DU spectrophotometer. In some instances (table 2) the initial methylene chloride fraction was evaporated to dryness … WebMar 16, 2012 · The reaction of urine can be tested by using simple litmus papers, or the reagent strips. Litmus papers: ... These are also known as Porter-silver chromogens. Principle and method: Porta-silber Reaction: The glucuronide conjugates of urinary corticosteroids are hydrolyzed with beta-glucoronidase. The freed steroids and free …
The Mechanism of the Porter—Silber Reaction. I.
WebThe Mechanism of the Porter—Silber Reaction. I. Rearrangement of the Dihydroxyacetone Group of Steroids1 Semantic Scholar Semantic Scholar extracted view of "The … WebPorter-Silber colour reaction. Thesecond method is the measurement of urinary 17-ketogenic steroids (Norymberski, Stubbs, and West, 1953). This is done by oxidizing the glucocorticoids and their metabolites to 17-ketosteroids with sodium bismuthate and estimating the newly formed and pre-existing 17-ketosteroids by the Zimmermann reaction. full back exercise routine
Evaluation of Silber-Porter Procedure for Determination of Plasma ...
WebMar 31, 2024 · In this study, four different colorimetric cortisol analyses that use various chromogens, which include sulfuric acid, Porter–Silber reagent, Prussian blue, and blue tetrazolium, are studied. Modifications to the classic Porter–Silber method are made by increasing the carbon content of the alcohol and adding gold nanoparticles, which result ... WebThis article is cited by 26 publications. Yibing Shi, Roumin Wang, Wei Yuan, Qingyun Liu, Mei Shi, Wei Feng, Zhiying Wu, Ke Hu, Fuyou Li. Easy-to-Use Colorimetric Cyanine Probe for the Detection of Cu2+ in Wilson’s Disease. Weba test, dependent on the Porter-Silber reaction, that is used as a measure of adrenocortical function and is performed on urine. Low values are seen in Addison disease and … gimnasio heron city