2024 Pslf172

Pslf172

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August undefined, 2024

WebPlasmid pREP3‐adh: The nmt1‐MCS‐3xHA‐term cassette from pSLF172 (Forsburg and Sherman, 1997) was cloned as a PstI/SacI fragment into pREP3X (gift of S.L. Forsburg), giving rise to plasmid pREP3X‐tag. Subsequently a XhoI/NotI fragment from pREP3X‐tag was substituted with a hybridized tw75/tw76 primer adapter giving pREP3‐SX. WebMar 17, 2014 · 2003). 3HA tag was fused in frame to the C-terminus of fission yeast Rec8 protein using pSLF172 (Forsburg & Sherman 1997). All strains used in this study are listed in Table S3 (Supporting Information). Synchronous meiotic cultures of budding and fission yeast and monitoring meiotic progression were conducted as described previously (Ohta …

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WebJul 4, 2024 · landed at Hartsfield-Jackson Intl - ATL. Saturday 04-Jul-2024 04:00PM CEST. (on time) Saturday 04-Jul-2024 07:20PM EDT. (15 minutes early) 9h 20m total travel time. … Webof pSLF172 (38). The orp4+ gene was synthesized by PCR and inserted into the Notl site of pRCE3X. Strain YRC4 (h-ura4-D18 pRCE3X-orp4N), expressing the N-terminal half of Orp4 … show me a picture of shiny

DNA: REPLICATION REPAIR AND RECOMBINATION - ScienceDirect

WebOct 1, 2024 · 4 beds, 2 baths, 1624 sq. ft. house located at 172 Longfellow Dr, Palm Springs, FL 33461 sold for $370,000 on Oct 1, 2024. MLS# RX-10646249. Modern, sleek, European … WebFeb 22, 2002 · All pSLF172 constructs express proteins in-frame with three C-terminal hemagglutinin (HA) epitope tags, which were detected by immunoblotting with the 12CA5 anti-HA monoclonal antibody (mAb). An insert containing ark1 + ::HA3 and the nmt1 terminator was subcloned from pSLF172- ark1 + into pJK210 using Xho I and Sac I to … WebThe hsk1 + open reading frame was amplified using PCR of S. pombe cDNA and cloned into pSLF172 ( 30 ) to give pSLF172-hsk1. The first 575 base pairs of the hsk1 + open reading frame, the nmt1 + promoter, and the ars1 sequences were removed from pSLF172-hsk1 by restriction enzyme cleavage and re-ligation. show me a picture of silver from sonic

The Fission Yeast Homologue of Orc4p Binds to Replication …

General purpose tagging vectors for fission yeast

WebClick here to find the location of this part on the equipment Part Number: 7017172, Description: Assembly, Counterbalance Valve, JLG Parts. Using genuine JLG parts is the … WebTo construct pSLF172 (L)-pka1 plasmid, the pka1 gene from the plasmid pSLF372(L)-pka1 was digested with the restriction enzymes BglII and NotI. The digested fragment was … show me a picture of shinglesWebJun 3, 1997 · As shown in Fig. 2 A, an anti-Cdc19p antibody recognizes a low amount of endogenous Cdc19p protein in the control lysate, and substantially increased amounts in … show me a picture of shrimp

"WebJan 15, 2002 · To construct the pSLF172-81X-sec31 expression plasmid, the sec31 ORF was first PCR amplified and cloned into the ura4 based plasmid pSLF173 (Forsburg and Sherman, 1997) to create pSLF173-sec31. A LEU2 marked, low-strength nmt1 -controlled expression plasmid was constructed by cleaving pSLF172 ( Forsburg and Sherman, 1997 … " - Pslf172

Pslf172

The Schizosaccharomyces pombe Aurora–related Kinase …

WebOct 28, 2005 · Next, GFP was isolated as an NcoI–NotI fragment from pcpy-GFP and ligated together with XhoI/NcoI digested PCR product and XhoI/NotI digested pSLF172, … WebELLULAR DNA replication requires precise regu- lation to ensure that the genome is replicated once completely in each cell cycle, and that the process of replication is coordinated with proper cell cycle entry and subsequent nuclear division.

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WebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. WebMay 10, 2002 · The plasmid pRCE81X was derived from the plasmid pREP81X (30) by replacing the 2.2-kb nmt1 promoter-terminator DNA fragment with thenmt1 promoter-terminator DNA fragment of pSLF172 (31). Theorc4 + gene was synthesized by PCR and inserted into the XhoI/NotI sites of pRCE81X, yielding pRCE81X-orc4.

WebpSLF172 87609 ™ Clone type Vector Applications Molecular biology Product format Freeze-dried See Additional Product Information Buy Now Price: $590.00 EA Discounts may be … WebPurification of Hsk1, a Minichromosome Maintenance Protein Kinase from Fission Yeast* (Received for publication, February 6, 1998, and in revised form, June 16, 1998)

Webproducts were digested with XhoI and BamHI, cloned into pSLF172, and transformed into S. pombe 201402 by electroporation. Transformed cells with the ura phenotype were … WebpSLF172,272,372 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially …

WebJun 10, 1997 · The source of Cig2 kinase, GBY228, is a strain carrying a plasmid expressing the cig2 + ORF fused at its C terminus to a triple hemaglutinin (HA) tag from the nmt1 …

WebJan 15, 2011 · To construct strains with tagged episomal versions of Cdc18 and Swi6, the cdc18 + open reading frame was amplified from S. pombe cDNA using PCR and cloned as a Xho1/Sma1 fragment into the pSLF172 vector 58 downstream of the nmt1 promoter, generating a Cdc18 tagged with the triple HA epitope sequence at the C-terminus. show me a picture of ski maskWebPcb-Power Supply; Led Tv Pd Bd, Pd46Cf2N_Zsm, Pslf172. Brand New. Available as a Special Order - Normal delay is 4 to 5 business days + shipping days. Order Now $ 253.98. Preorder. Qty. Questions ? Ask us About This Product. More Details; Warranty; COMPATIBLE MODELS. Samsung UA46C7000WRMXL. 90 Day Factory Warranty ... show me a picture of skaiWebThe S. pombe expression vector pREP3X, pSLF172, and pSLF173 series were obtained from recombinant DNA vec-tors in the American Type Culture Collection (ATCC) (Table 1). To construct fluorescent protein-tagged vectors, the coding sequences of the fluorescent proteins were cloned into the S. pombe autonomously replicating sequence (ARS) element- show me a picture of skinWebepitope in its C-terminal region. To construct pSLF172(L)-pka1, the pka1 gene from pSLF372(L)-pka1 was digested with BglII and NotI. The digested fragment was cloned … show me a picture of silverfishWebof pSLF172 (38). The orp41 gene was synthesized by PCR and inserted into the NotI site of pRCE3X. Strain YRC4 (h2 ura4-D18 pRCE3X-orp4N), expressing the N-terminal half of Orp4 … show me a picture of skittlesWebof pSLF172 (38). The orp41 gene was synthesized by PCR and inserted into the NotI site of pRCE3X. Strain YRC4 (h2 ura4-D18 pRCE3X-orp4N), expressing the N-terminal half of Orp4 protein (amino acids 1–486), and strain YRC5 (h2 ura4-D18 pRCE3X-orp4C), expressing the C-terminal terminal half of Orp4 protein (amino acids 436–972), were constructed show me a picture of skinwalkerWebOct 13, 2024 · Thecdc19-M4 mutant was constructed and cloned into the HA-tagging nmt1 + expression vector pSLF172 (Forsburg and Sherman, 1997) as described previously (Forsburg et al., 1997). Plasmid pMF56, expressing N-terminally HA-tagged Cdc18p under control of the nmt1 + promoter was a kind gift of Marco Muzi-Falconi and Tom Kelly (Muzi … show me a picture of sign language