Tīmeklis5 Figure S2. NG2+ cells express QK and loss of Qk abolishes NG2 cells; relating to Figures 2, 3, and 4 (A) Co-Immunostaining of QK (red) and CNP1 (green) in the coronal sections of P0 control (Cont) cortex. Scale bar = 50 μm. (B) Co-Immunostaining of QK (red) and CNP1 (green) in the coronal sections of P7 Cont and Qk-KO cortices.The … Tīmeklis2016. gada 12. maijs · Of these 23 rare codons, 11 codons appear in the first 106 codons, which encode the propeptide sequence. Both the wild-type Subtilisin QK-2 gene (qk) and the codon-optimized gene (qk′ and qkpro′) were expressed in the same way, and the results were used to study the effect of codon optimization on heterologous …
Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system …
Tīmeklis2024. gada 13. okt. · Functional analysis of the NSC-specific Qk-null mutant mouse revealed the key role of Qk in astrocyte and oligodendrocyte generation and … Tīmeklis2016. gada 12. maijs · The biological activity of this secreted Subtilisin QK-2 was enhanced by modulating the pH of medium to slightly alkaline during induction and by codon optimization of either the entire gene ... diy beach cart tires
The STAR protein QKI-6 is a translational repressor PNAS
Tīmeklis2011. gada 17. janv. · In Arabidopsis, there are four homologs of class A1 heat shock factor (HSFA1) genes, which likely encode the master regulators of heat shock response (HSR).However, previous studies with double knockout (KO) mutants were unable to confirm this point probably due to functional redundancy. Here, we … Tīmeklis2016. gada 12. maijs · The biological activity of this secreted Subtilisin QK-2 was enhanced by modulating the pH of medium to slightly alkaline during induction and … Tīmeklis2024. gada 1. marts · The total length of the QK gene is 1128 bp.For expression in P. pastoris, 1062 bp of the gene was required, which encoded 353 amino acids .To improve the expression efficiency of the recombinant protein, the DNA sequence encoding QK was designed and synthesized based on the codon bias of P. … crafty things