WebJul 26, 2024 · 1 . detected in THP -1 cells with or without synthetic receptors. 2 Antibody-mediated phagocytosis and internalization of are important virions 3 mechanisms of … WebAug 8, 2015 · Based on the literature, we tested different concentrations of LPS, varying from 1 to 100 ng/ml, combined with 20 ng/ml of IFN-γ and we incubated THP-1 macrophages …
Macrophage-like THP-1 cells show effective uptake of silica
WebSo RTX-CD47 is more effective than anti-CD47 antibody in the regulation of innate immune cells by inhibiting CD47-SIRPα on CD47-positive cancer cells with better macrophage-mediated phagocytosis assay. 68 Anti-CD47 antibody therapy combined with chemotherapy DOX could regulate autophagy and prevent cardiac toxicity to inhibit invasive breast … WebMIT inhibited the surface CD47 expression and promoted the phagocytosis of SK-MEL-28 cells by THP-1 cells. We found MIT inhibited tumor growth in melanoma allograft mice and CD47 expression in tumor mass. We also found MIT upregulated PD-L1 expression in cancer cells possibly via inhibiting PD-L1 ubiquitination, increasing ROS and IFN-γ. howden claims
flow cytometric assay to measure the modulation of phagocytosis
Web1 day ago · FcγR expression of THP-1-CD16A macrophages was 168 evaluated by flow cytometry using fluorescent antibodies to FcγRI (clone 10.1), FcγRIIA 169 (clone IV.3), FcγRIIA/B/C (clone AT10), and FcγRIIIA/B (clone 3G8). 170 In vitro macrophage phagocytosis of ITP serum-opsonized platelets 171 Human macrophage phagocytosis … WebOct 15, 2013 · SIRPα, an ITIMs-containing signaling receptor, negatively regulates leukocyte responses through extracellular interactions with CD47. However, the dynamics of SIRPα-CD47 interactions on the cell surface and the governing mechanisms remain unclear. Here we report that while the purified SIRPα binds to CD47 and that SIRPα is expressed on … WebMar 18, 2024 · BMDMs pretreated with DMSO or paclitaxel were used for the assay. Phagocytosis assay was performed in the presence of cetuximab (DLD1 and SW480 … how many regular size tea bags 1 family bag